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Addgene inc pmal c2x addgene
Pmal C2x Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pmal+c2x+addgene/pMAL-c2X+(Plasmid+%2375286)/pm38870008-146-169-170
Average 95 stars, based on 50 article reviews

pmal c2x addgene - by Bioz Stars, 2026-09

95/100 stars

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Chromatin Immunoprecipitation:

Article Title: Increased ACTL6A occupancy within mSWI/SNF chromatin remodelers drives human squamous cell carcinoma.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 3X FLAG Peptide MilliporeSigma Cat# F4799-4MG Dynabeads Protein G Thermo Fisher Scientific Cat# 10009D Geltrex Thermo Fisher Scientific Cat# A1413302 Transferrin Roche Cat# 10652202001 EGF Thermo Fisher Scientific Cat# PHG0311 Insulin Sigma-Aldrich Cat# I5500 Forskolin Tocris Bioscience Cat# 1099 VX-745 Tocris Bioscience Cat# 3915 RO4929097 Cellagen Technology Cat# C7649 Dexamethasone Tocris Bioscience Cat# 1126 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140-163 Deoxyribonuclease I Worthington Biochemical Corporation Cat# LS002058 TRIsure Bioline Cat# BIO-38033 Plasmocin InvivoGen Cat# ant-mpp Doxycycline Sigma-Aldrich Cat# D9891 Fibronectin Sigma-Aldrich Cat# F1141-1MG DharmaFECT 1 transfection reagent Horizon Discovery Cat# T-2001-01 5X siRNA Buffer Horizon Discovery Cat# B-002000-UB-100 Puromycin dihydrochloride Sigma-Aldrich Cat# P8833-100mg ON-TARGETplus Human ACTL6A siRNA Horizon Discovery Cat# L-008243-00 ON-TARGETplus Non-targeting Pool Horizon Discovery Cat# D-001810-10 Blasticidin S HCl Thermo Fisher Scientific Cat# R21001 Critical commercial assays NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat# E7490S NEBNext MultiplexOligos for Illumina (Dual Index Primers Set 1) New England Biolabs Cat# E7600S NEBNext High-Fidelity 2X PCR Master Mix New England Biolabs Cat# M0541S Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q32854 ZymoPURE II Plasmid Midiprep Kit Zymo Research Cat# D4200 DNA Clean & Concentrator-5 Zymo Research Cat# D4013 Direct-zol RNA Miniprep Kits Zymo Research Cat# R2050 High Sensitivity D1000 Reagents (Sample Buffer & Ladder) Agilent Technologies Cat# 5067-5585 High Sensitivity D1000 ScreenTapes Agilent Technologies Cat# 5067-5584 NextSeq 500/550 High Output Kit v2.5 (75 Cycles) Illumina Cat# 20024906 Bio-Rad Protein Assay Dye (Bradford) Bio-Rad Cat# 500-0006 Lenti-X GoStix Clontech Cat# 631281 PhiX Control v3 Illumina Cat# FC-110-3001 SensiFAST SYBR Lo-ROX Kit Bioline Cat# BIO-94020 SensiFAST cDNA Synthesis Kit Bioline Cat# BIO-65054 KLD Enzyme Mix New England Biolabs Cat# M0554S In-Fusion HD Cloning Kit Clontech Cat# 639650 NEBNext Ultra II DNA Library Prep with Sample Purification Beads New England Biolabs Cat# E7103S NEBNext Ultra II Directional RNA Library Prep Kit New England Biolabs Cat# E7760S Deposited data Structure of nucleosome-bound human BAF complex Protein Data Bank PDB: 6LTJ Deep sequencing datasets from this study Gene Expression Omnibus GEO: GSE156788 (Continued on next page) e2 Molecular Cell 81, 4964–4978.e1–e8, December 16, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER H3K4me3 ChIP-seq (keratinocytes) ENCODE Dataset: ENCSR075OQB H3K27me3 ChIP-seq ENCODE Dataset: ENCSR377MRR Original image data Mendeley Mendeley data: http://dx.doi. org/10.17632/gsd3wvy3nw.1 Experimental models: Cell lines Human Epidermal Keratinocytes, adult (HEKa) Thermo Fisher Scientific Cat# C0055C HEK293T Takara Cat# 632180 FaDu ATCC Cat# HTB-43 NCI-H520 ATCC Cat# HTB-182 T.T JCRB Cell Bank Cat# JCRB0262 KYSE70 Sigma Cat# 94072012 Oligonucleotides Primers See Table S1 N/A Recombinant DNA pQCXIH-Myc-YAP Addgene Cat# 33091 CYC244-Flag-NLS-hYAP This study N/A N106-hACTL6A This study N/A CYC103-hACTL6A This study N/A pGSTag Addgene Cat# 21877 pMAL-c2X Addgene Cat# 75286 lentiCRISPR v2 Addgene Cat# 52961 psPAX2 Addgene Cat# 12260 pMD2.G Addgene Cat# 12259 Software and algorithms Adobe Creative Cloud Adobe https://www.adobe.com/creativecloud.html Rstudio RStudio https://www.rstudio.com/ Image Studio Lite LI-COR https://www.licor.com/bio/ SnapGene Insightful Science https://www.snapgene.com/ Prism 8 GraphPad Software https://www.graphpad.com/ scientific-software/prism/ SeqPurge Sturm et al., 2016 N/A Bowtie2 Langmead and Salzberg, 2012 N/A deeptools Ramı́rez et al., 2016 N/A macs2 Zhang et al., 2008 N/A Homer Heinz et al., 2010 N/A DEseq2 Love et al., 2014 N/A edgeR Robinson et al., 2010 N/A DiffBind Ross-Innes et al., 2012 N/A chromVar Schep et al., 2017 N/A ChiPseeker Yu et al., 2015b N/A ChrAccR https://github.com/ GreenleafLab/ChrAccR N/A PyMOL v2.4.2 Schrodinger https://pymol.org/2/ .. REAGENT or RESOURCE SOURCE IDENTIFIER H3K4me3 ChIP-seq (keratinocytes) ENCODE Dataset: ENCSR075OQB H3K27me3 ChIP-seq ENCODE Dataset: ENCSR377MRR Original image data Mendeley Mendeley data: http://dx.doi. org/10.17632/gsd3wvy3nw.1 Experimental models: Cell lines Human Epidermal Keratinocytes, adult (HEKa) Thermo Fisher Scientific Cat# C0055C HEK293T Takara Cat# 632180 FaDu ATCC Cat# HTB-43 NCI-H520 ATCC Cat# HTB-182 T.T JCRB Cell Bank Cat# JCRB0262 KYSE70 Sigma Cat# 94072012 Oligonucleotides Primers See Table S1 N/A Recombinant DNA pQCXIH-Myc-YAP Addgene Cat# 33091 CYC244-Flag-NLS-hYAP This study N/A N106-hACTL6A This study N/A CYC103-hACTL6A This study N/A pGSTag Addgene Cat# 21877 pMAL-c2X Addgene Cat# 75286 lentiCRISPR v2 Addgene Cat# 52961 psPAX2 Addgene Cat# 12260 pMD2.G Addgene Cat# 12259 Software and algorithms Adobe Creative Cloud Adobe https://www.adobe.com/creativecloud.html Rstudio RStudio https://www.rstudio.com/ Image Studio Lite LI-COR https://www.licor.com/bio/ SnapGene Insightful Science https://www.snapgene.com/ Prism 8 GraphPad Software https://www.graphpad.com/ scientific-software/prism/ SeqPurge Sturm et al., 2016 N/A Bowtie2 Langmead and Salzberg, 2012 N/A deeptools Ramı́rez et al., 2016 N/A macs2 Zhang et al., 2008 N/A Homer Heinz et al., 2010 N/A DEseq2 Love et al., 2014 N/A edgeR Robinson et al., 2010 N/A DiffBind Ross-Innes et al., 2012 N/A chromVar Schep et al., 2017 N/A ChiPseeker Yu et al., 2015b N/A ChrAccR https://github.com/ GreenleafLab/ChrAccR N/A PyMOL v2.4.2 Schrodinger https://pymol.org/2/

Recombinant:

Software:

other:

Article Title: 53BP1 Supports Immunoglobulin Class Switch Recombination Independently of Its DNA Double-Strand Break End Protection Function
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER CellTrace Violet Thermofisher Cat# C34557 Paraformaldehyde Sigma-Aldrich Cat# P6148 Hoechst 33258 Thermofisher Cat# H3569 Neocarzinostatin (NCS) Sigma Cat# N9162 16% Formaldehyde Methanol-free Thermo scientific Cat# 28908 Dynabeads Protein G ThermoFisher Cat# 10004D FuGENE HD Transfection Reagent Promega Cat# E2312 Igepal CA-630 Sigma Cat# I8896 Pierce Phosphatase Inhibitor Mini Tablets ThermoFisher Cat# A32957 Phenylmethylsulfonyl fluoride Sigma-Aldrich Cat# 10837091001 Pepstatin A Merck Cat# P5318 Critical Commercial Assays APC BrdU Flow Kit BD Biosciences Cat# 552598 Neon Transfection System, 100 ml Kit Invitrogen Cat# MPK10025 Expand Long Template PCR System Sigma-Aldrich Cat# 11681834001 NucleoSpin DNA Purification Kit Macherey-Nagel Cat# 740499 TOPO TA Cloning Kit Invitrogen Cat# 450641 Luna Universal One-Step RT-qPCR Kit NEB Cat# E3005S Deposited Data Original scans and IF images This paper; Mendeley Data https://data.mendeley.com/datasets/ n7jxbpddnf/draft?a=d8838dbd-c9e940d5-b467-0150330356a1 Experimental Models: Cell Lines Murine: CH12 (CH12F3) - WT, parental Nakamura et al., 1996 N/A Murine: 53bp1 / CH12 Delgado-Benito et al., 2018 N/A Murine: 53bp1D6 CH12 This paper N/A Murine: 53bp1 / iMEFs Bothmer et al., 2011 N/A Murine: WT iMEFs Bothmer et al., 2011 N/A Human: BOSC23 Pear et al., 1993 N/A Experimental Models: Organisms/Strains Mouse: 53bp1 / (and derived splenocytes cultures) Ward et al., 2003 N/A Oligonucleotides 53BP1DBRCT-3xHA construct cloning See Table S2 N/A 53BP1DPRO- & 53BP1DMOB-3xFlag construct cloning See Table S2 N/A CRISPR-Cas9 gene targeting See Table S2 N/A RPA ChIP-qPCR See Table S2 N/A End resection assay See Table S2 N/A Switch junctional analysis See Table S2 N/A Recombinant DNA Plasmid: pET21b+ Novagen Cat# 69741 Plasmid: pMAL-C2X Addgene Cat# #75286 Plasmid: pMX-53BP1DBRCT-3xFlag Bothmer et al., 2011 N/A Plasmid: pMX-53BP1DOligo-3xFlag Ward et al., 2006 N/A Plasmid: pMX-53BP1DCore-3xFlag This paper N/A Plasmid: pMX-53BP1D1256A-3xFlag Zgheib et al., 2009 N/A Plasmid: pMX-53BP1mTudor-3xFlag Botuyan et al., 2006 N/A Plasmid: pMX-53BP128A-3xFlag Bothmer et al., 2011 N/A Plasmid: pMX-53BP1DPro-3xFlag This paper N/A (Continued on next page) Cell Reports 28, 1389–1399.e1–e6, August 6, 2019 e2

Article Title: Wg/Wnt-signaling-induced nuclear translocation of β-catenin is attenuated by a β-catenin peptide through its interference with the IFT-A complex.
Article Snippet: We thank the Bloomington Drosophila Stock Center for fly strains and Developmental Studies Hybridoma Bank for antibodies.

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